ProductOverview
Theundifferentiatedstateofembryonicstem(ES)andinducedpluripotentstem(iPS)cellscanbecharacterizedbyahighlevelofalkalinephosphatase(AP)expressionwhich,alongwiththeexpressionofothersurfaceMarkers,indicatesundifferentiatedcellswiththepotentialtoself-renew.APisahydrolaseenzymeresponsIBLefordephosphorylatingmoleculessuchasnucleotides,proteins,andalkaloidsunderalkalineconditions.WhenfixedESoriPScellsarestainedusingtheAPStainingKit,undifferentiatedcellsappearredorpurple,whereasdifferentiatedcellsappearcolorless.
ProductSpecifications
Size
50assays
KitComponents
FixSolution:25ml(Cat.No.09-0042)
APStainingSolutionA:10ml(Cat.No.09-0043)
APStainingSolutionB:10ml(Cat.No.09-0065)
APStainingSolutionC:10ml(Cat.No.09-0066)
StorageandStABIlity
Storeat4°C.Donotfreeze.Stablefor6monthsfromdateofreceiptwhenstoredasdirected.
QualityControl
TheAPStainingKitIIisfunctionallytestedonhumanandmouseEScellstoensureproductquality.
SpecificationSheets
- 00-0055SpecificationsSheet
SafetyDataSheets
- 00-0055SafetyDataSheet
- 09-0042SafetyDataSheet
- 09-0043SafetyDataSheet
- 09-0065SafetyDataSheet
- 09-0066SafetyDataSheet
Protocols
- Protocol:AlkalinePhosphataseStainingKitII
References
- Pease,S.,Braghetta,P.,Gearing,D.,Grail,D.,andWilliams,R.L.(1990)Isolationofembryonicstem(ES)cellsinmediasupplementedwithrecombinantleukemiainhibitoryfactor(LIF).DevBiol.141:344-352.
- Smith,A.G.,Nichols,J.,Robertson,M.,andRathjen,P.D.(1992)Differentiationinhibitingactivity(DIA/LIF)andmousedevelopment.Dev.Biol.151:339-351.
- Takahashi,K.,TanabeK.,Ohnuki,M.,Narita,M.,IchisakaT.,TomodaK.andYamanaka,S.(2007)Inductionofpluripotentstemcellsfromadulthumanfibroblastsbydefinedfactors.Cell131:861-872.
- Yu,J.,Vodyanik,M.A.,Smuga-Otto,K.,Antosiewicz-Bourget,J.,Frane,J.L.,Tian,S.,Nie,J.,Jonsdottir,G.A.,Ruotti,V.,Stewart,R.,Slukvin,I.I.,andThomson,J.A.(2007)Inducedpluripotentstemcelllinesderivedfromhumansomaticcells.Science318:1917-1920.
AdditionalPublications
- MomcilovicO;SivapathamR;OronTR;MeyerM;MooneyS;RaoMA;ZengX."Derivation,characteriazation,andneuraldifferentiationofintegration-freeinducedpluripotentstemcelllinesfromParkinson"sdiseasepatientscfarryingSNCA,LRRK2,PARK2,andGBAmutations."PLoSONE11(5):e0154890.doi:10.1371/journal.pone.0154890(2016)
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