ProductOverview
Stage-specificembryonicantigen-1(SSEA-1),alsoknownasCD15,isanantigenicepitopedefinedasaLewisXcarbohydratestructure.Itisexpressedonmurineembryonalcarcinoma(EC)cells,murineembryonicstem(ES)cells,andmurineandhumangermcells.SSEA-1iswidelyusedasapositivesurfaceMarkerformouseundifferentiatedstemcellsandanegativesurfacemarkerforhumanundifferentiatedstemcells.Expressionisdown-regulatedfollowingdifferentiationofmurineECandEScells,whilethedifferentiationofhumanECandEScellsisaccompaniedbyanincreaseinSSEA-1expression.SSEA-1isassociatedwithcelladhesion,migration,anddifferentiation.Foruseonlivecells,thisantibodyhasbeenspecificallyformulatedwithlowendotoxinandnosodiumazide.EScellmorphology,proliferation,andexpressionofpluripotencymarkersdoesnotchangeafterstainingwiththisantibody.
ProductSpecifications
Size
100μl
Concentration
0.5mg/ml
Clone
MC-480
Isotype
MouseIgM,κ
Immunogen
MouseF9teratocarcinomastemcells(X-irrADIated)
Reactivity
Mouse,Human
Preparation
ThisantibodywaspurifiedbyaffinitychromatographyandconjugatedwithDyLight550underoptimalconditions.Thesolutionisfreeofunconjugateddyeandunlabeledantibody.Theformulationis0.2μmfiltersterilizedandfreefrombacteria,fungi,mycoplasma,andvirus.
Formulation
Phosphate-bufferedsolution,pH7.2,containingnopreservative
StorageandStABIlity
Storeat4°Cprotectedfromlight.Donotfreeze.Handleunderasepticconditions.Stablefor6monthsfromdateofreceiptwhenstoredasdirected.
QualityControl
Testedbyimmunocytochemistrytoensureproductquality.
Endotoxinlevelislessthan0.1EU/μg(0.01ng/μg)oftheproteinasdeterminedbytheLALassay.
RecommendedUsage
Thesuggesteduseofthisantibodyisa1:100dilutionforimmunocytochemistry.Foranapplicationspecificprotocol,pleasereferenceProtocol:ImmunocytochemistryofLiveCellsonlineatwww.Stemgent.com/support/protocols.
SpecificationSheets
- 09-0095SpecificationsSheet
SafetyDataSheets
- 09-0095SafetyDataSheet
Protocols
- GeneralProtocolforICCStainingofLiveCells
References
- Brambrink,T.,Foreman,R.,Welstead,G.G.,Lengner,C.J.,Wernig,M.,Suh,H.,andJaenisch,R.(2008)Sequentialexpressionofpluripotencymarkersduringdirectreprogrammingofmousesomaticcells.CellStemCell2:151-159.
- Fox,N.,Damjanov,I.,Martinez-Hernandez,A.,Knowles,B.B.,andSolter,D.(1981)Immunohistochemicallocalizationoftheearlyembryonicantigen(SSEA-1)inpostimplantationmouseembryosandfetalandadulttissues.DevBiol83:391-398.
- Andrews,P.W.,Goodfellow,P.N.,Shevinsky,L.,Bronson,D.L.,andKnowles,B.B.(1982)Cellsurfaceantigensofaclonalhumanembryoniccarcinomacellline:morphologicalandantigenicdifferentiationinculture.Int.J.Cancer29:523-531.
- Draper,J.S.,Pigott,C.,Thomson,J.A.,andAndrews,P.W.(2002)Surfaceantigensofhumanembryonicstemcells:changesupondifferentiationinculture.J.Anat.200:249-258.
- Cui,L.,Johkura,K.,Yue,F.,Ogiwara,N.,Okouchi,Y.,Asanuma,K.,andSasaki,K.(2004)SpatialdistributionandinitialchangesofSSEA-1andothercelladhesion-relatedmoleculesonmouseembryonicstemcellsbeforeandduringdifferentiation.JHistochemCytochem52:1447-1457.
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