ProductOverview
Stage-specificembryonicantigen-4(SSEA-4)isexpressedinavarietyofhumancelllinesincludingembryoniccarcinoma(EC),embryonicstem(ES),andinducedpluripotentstem(iPS)cells.SSEA-4isdown-regulatedupondifferentiation,makingitawidelyusedMarkertocharacterizehumanEScellsandmonitortheirdifferentiation.Inmouse,SSEA-4isexpressedonoocytesandearlycleavage-stageembryos,butnotonECorEScells.Foruseonlivecells,thisantibodyhasbeenspecificallyformulatedwithlowendotoxinandnosodiumazide.EScellmorphology,proliferation,andexpressionofpluripotencymarkersdoesnotchangeafterstainingwiththisantibody.
ProductSpecifications
Size
100μl
Concentration
0.5mg/ml
Clone
MC-813-70
Isotype
MouseIgG3,κ
Immunogen
Humanembryonalcarcinomacellline2102Ep
Reactivity
Human
Preparation
ThisantibodywaspurifiedbyaffinitychromatographyandconjugatedwithDyLight550underoptimalconditions.Thesolutionisfreeofunconjugateddyeandunlabeledantibody.Theformulationis0.2µmfiltersterilizedandfreefrombacteria,fungi,mycoplasma,andvirus.
Formulation
Phosphate-bufferedsolution,pH7.2,containingnopreservative
StorageandStABIlity
Storeat4°Cprotectedfromlight.Donotfreeze.Handleunderasepticconditions.Stablefor6monthsfromdateofreceiptwhenstoredasdirected.
QualityControl
Testedbyimmunocytochemistrytoensureproductquality.Endotoxinlevelislessthan0.1EU/µg(0.01ng/µg)oftheproteinasdeterminedbytheLALassay.
RecommendedUsage
Thesuggesteduseofthisantibodyisa1:100dilutionforimmunocytochemistry.
SpecificationSheets
- 09-0097SpecificationsSheet
SafetyDataSheets
- 09-0097SafetyDataSheet
Protocols
- GeneralProtocolforICCStainingofLiveCells
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