ProductOverview
NanogisamemberofthehomeoboxfamilyofDNAbindingtranscriptionfactorsthathasbeenshowntomaintainembryonicstem(ES)cellself-renewalindependentlyofleukemiainhibitoryfactor(LIF)andStat3.NanogmRNAispresentinpluripotentmouseandhumancelllinesandisabsentfromdifferentiatedcells.Functionally,Nanogworkstogetherwithotherkeypluripotentfactors(Oct4,Sox2,andLin28)toreprogramhumanfibroblastsandgenerateinducedpluripotentstem(iPS)cells.Thesekeyfactorsformaregulatorynetworktosupportorlimiteachother'sexpressionlevel,maintainingthepropertiesofEScells.TheNanogAntibody(AffinityPurified)wasscreenedonhumanandmouseEScellsusingimmunocytochemistryandselectedasthebestNanogantibodyavailableforresearchersneedingtodemonstratepluripotency.
ProductSpecifications
Size
100μl
Concentration
0.5mg/ml
Clone
Polyclonal
Isotype
RabbitIgG
Immunogen
Recombinantproteinfragmentcontainingasequencecorrespondingtoaregionwithinaminoacids109and300ofhumanNanog
Reactivity
Mouse,Human
Preparation
Thisantibodywaspurifiedby antigenaffinitychromatography.
Formulation
Phosphate-bufferedsolution,pH7.2,1%BSA,20%glycerol,and0.01%thimerosal
StorageandStABIlity
Storeat4˚Cprotectedfromlight.Donotfreeze.Stablefor6monthsfromdateofreceiptwhenstoredasdirected.
QualityControl
Testedbyimmunocytochemistrytoensureproductquality.
RecommendedUsage
Forimmunocytochemistry,thesuggesteduseofthisantibodyisa1:100dilution.Foranapplicationspecificprotocol,pleasereferenceProtocol:Immunocytochemistryonlineatwww.Stemgent.com/support/protocols.
SpecificationSheets
- 09-0020SpecificationsSheet
SafetyDataSheets
- 09-0020SafetyDataSheet
Protocols
- Protocol:Immunocytochemistry
RelatedProducts
- mRNAReprogrammingKit(00-0071)
- microRNABoosterKit(00-0073)
References
- Mitsui,K.,Tokuzawa,Y.,Itoh,H.,Segawa,K.,Murakami,M.,Takahashi,K.,Maruyama,M.,Maeda,M.,andYamanaka,S.(2003)ThehomeoproteinNanogisrequiredformaintenanceofpluripotencyinmouseepIBLastandEScells.Cell113:631-642.
- Chambers,I.,Colby,D.,Robertson,M.,Nichols,J.,Lee,S.,Tweedie,S.,andSmith,A.(2003)FunctionalexpressioncloningofNanog,apluripotencysustainingfactorinembryonicstemcells.Cell113:643-655.
- Yu,J.,Vodyanik,M.A.,Smuga-Otto,K.,Antosiewicz-Bourget,J.,Frane,J.L.,Tian,S.,Nie,J.,Jonsdottir,G.A.,Ruotti,V.,Stewart,R.,Slukvin,I.I.,andThomson,J.A.(2007)Inducedpluripotentstemcelllinesderivedfromhumansomaticcells.Science318:1917-1920.
- Pan,G.,andThomson,J.A.(2007)Nanogandtranscriptionalnetworksinembryonicstemcellpluripotency.CellRes.17:42-49.
- Kim,J.,Chu,J.,Shen,X.,Wang,J.,andOrkin,S.H.(2008)Anextendedtranscriptionalnetworkforpluripotencyofembryonicstemcells.Cell132:1049-1061.
AdditionalPublications
- PoleganovMA;EminliS;BeissertT;HerzS;MoonJ-I;GoldmannJ;BeyerA;HeckR;BurkhartI;RoldanDB;TureciO;YiK;HamiltonB;SahinU."Efficientreprogrammingofhumanfibroblastsandblood-derivedendothelialProgenitorcellsusingnonmodifiedRNAforreprogrammingandimmuneevasion."HumanGeneTherapy26:751(2015)
- RenJ;BrionesV;BarbourS;YuW;HanY;TarashimaM;MueggeK."TheATPbindingsiteofthechromatinremodelinghomologLSHisrequiredfornucleosomedensityanddenovoDNAmethylationatrepeatsequences."NuclAcidsResdoi:10.1093/nar/gku1371(2015)
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