ProductOverview
Oct4(Octamer-4,alsoknownasPOU5F1)isahomeodomaintranscriptionfactorofthePOUfamilywhichisinvolvedintheregulationofpluripotencyduringnormaldevelopment.TheexpressionofOct4isassociatedwithanundifferentiatedphenotypeinembryonicstem(ES)cells,whilegeneknockdownofOct4promotesdifferentiation.Assuch,itisfrequentlyusedasaMarkerforundifferentiatedEScellsorinducedpluripotentstem(iPS)cells.Oct4isoneofthekeytranscriptionfactorsusedtoreprogrammouseandhumanfibroblaststoapluripotentstate.TheOct4Antibody(AffinityPurified)wasscreenedonhumanandmouseEScellsusingimmunocytochemistryandflowcytometryandselectedasthebestOct4antibodyavailableforresearchersneedingtodemonstratepluripotency.
ProductSpecifications
Size
100μl
Clone
Polyclonal
Isotype
RabbitIgG
Immunogen
SyntheticpeptideconjugatedtoKLHderivedfromwithinresidues300totheC-terminusofhumanOct4
Reactivity
Mouse,Human
Preparation
Theantibodywaspurifiedbyimmunogenaffinitychromatography.
Formulation
Phosphate-bufferedsolution,pH7.4,1%BSAand0.02%sodiumazide
StorageandStABIlity
Storeat4°Cprotectedfromlight.Donotfreeze.Stablefor6monthsfromdateofreceiptwhenstoredasdirected.
QualityControl
Testedbyimmunocytochemistryandflowcytometrytoensureproductquality.
RecommendedUsage
Thesuggesteduseofthisantibodyisa1:100dilutionforimmunocytochemistryanda1:5dilutionforflowcytometry.Forapplicationspecificprotocols,pleasereferenceProtocol:ImmunocytochemistryandProtocol:FlowCytometryonlineatwww.Stemgent.com/support/protocols.
SpecificationSheets
- 09-0023SpecificationsSheet
SafetyDataSheets
- 09-0023SafetyDataSheet
Protocols
- Protocol:ImmunoflourescenceStainingofCellsforFlowCytometry
- Protocol:Immunocytochemistry
RelatedProducts
- mRNAReprogrammingKit(00-0071)
- microRNABoosterKit(00-0073)
References
- Looijenga,L.H.,Stoop,H.,deLeeuw,H.P.,deGouveiaBrazao,C.A.,Gillis,A.J.,vanRoozendaal,K.E.,vanZoelen,E.J.,Weber,R.F.,Wolffenbuttel,K.P.,vanDekken,H.,Honecker,F.,Bokemeyer,C.,Perlman,E.J.,Schneider,D.T.,Kononen,J.,Sauter,G.,Oosterhuis,J.W.(2003)POU5F1(OCT3/4)identifiescellswithpluripotentpotentialinhumangermcelltumors.CancerRes63:2244-2250.
- Zaehres,H.,Lensch,M.W.,Daheron,L.,Stewart,S.A.,Itskovitz-Eldor,J.,Daley,G.Q.(2005)High-efficiencyRNAinterferenceinhumanembryonicstemcells.StemCells23:299-305.
- Takahashi,K.,andYamanaka,S.(2006).Inductionofpluripotentstemcellsfrommouseembryonicandadultfibroblastculturesbydefinedfactors.Cell126,663-676.
- Takahashi,K.,TanabeK.,Ohnuki,M.,Narita,M.,IchisakaT.,TomodaK.andYamanaka,S.(2007)Inductionofpluripotentstemcellsfromadulthumanfibroblastsbydefinedfactors.Cell131:861-872.
- Park,I.H.,Arora,N.,Huo,H.,Maherali,N.,Ahfeldt,T.,Shimamura,A.,Lensch,M.W.,Cowan,C.,Hochedlinger,K.,andDaley,G.Q.(2008)Disease-specificinducedpluripotentstemcells.Cell134:877-886.
AdditionalPublications
- BurnettLC;LeDucCA;SulsonaCR;PaullD;EddiraS;LevyB;SallesJP;TauberM;DriscollDJ;EgliD;LeibelRL."Inducedpluripotentcells(iPSC)createdfromskinfibroblastaofpatientswithPrader-Willisyndrome(PWS)retainthemolecularsignatureofPWS."StemCellRes(2016),doi:10.1016/j.scr.2016.08.008
- SridharA;OhlemacherSK;LangerKB;MeyerJS."RobustdifferentiationofmRNA-reprogrammedhumaninducedpluripotentstemcellstoardaretinallineage."StemCellTranslationalMedicine5:10(2016)
- OhlemacherSK;SridharA;XiaoY;HochstetlerAE;SarfaraziM;CumminsTR;MeyerJS."Stepwisedifferentiationofretinalganglioncellsfromhumanpluripotentstemcellsenablesanalysisofglaucomatousneurodegeneration."StemCellsdoi:10.1002/stem.2356(2016)
- PoleganovMA;EminliS;BeissertT;HerzS;MoonJ-I;GoldmannJ;BeyerA;HeckR;BurkhartI;RoldanDB;TureciO;YiK;HamiltonB;SahinU."Efficientreprogrammingofhumanfibroblastsandblood-derivedendothelialProgenitorcellsusingnonmodifiedRNAforreprogrammingandimmuneevasion."HumanGeneTherapy26:751(2015)
- HanF;WangW;CHenB;CHenC;LISen;LuX;DuanJ;ZhangY;ZhangYA;GuoW;LiG."Humaninducedpluripotentstemcell-derivedneuronsimprovemotorasymmetryina6-hydroxydopamine-inducedratmodelofParkinson"sdisease."Cytotherapy17:665(2015)
- PaullD;SevillaA;ZhouH;HahnAK;KimH;NapolitanoC;TsankovA;ShangL;KrumholzK;JagaseesanP;WoodardCM;SunB;VilbouxT;ZimmerM;ForeroE;MoroziewiczDN;MartinezH;MalicdanMCV;WeissKA;VensandLB;DusenberryCR;PolusH;SyKTL;KahlerDJ;GahlWA;SolomonSL;ChangS;MeissnerA;EgganK;NoggleSA."Automated,high-throughputderivation,characterizationanddifferentiationofinducedpluripotentstemcells."NaureMethods12:885(2015)
- RenJ;BrionesV;BarbourS;YuW;HanY;TarashimaM;MueggeK."TheATPbindingsiteofthechromatinremodelinghomologLSHisrequiredfornucleosomedensityanddenovoDNAmethylationatrepeatsequences."NuclAcidsResdoi:10.1093/nar/gku1371(2015)
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