ProductOverview
TheMC-631monoclonalantibodyreactswithstage-specificembryonicantigen-3(SSEA-3),acarbohydrateepitopeonthemajorgangliosidethatisexpresseduponthesurfaceofhumanembryonalcarcinoma(EC)cells,embryonicgerm(EG)cells,andembryonicstem(ES)cells.TheexpressionofSSEA-3onhumanEScellsisdown-regulatedupondifferentiation.Inmouse,SSEA-3isexpressedonunfertilizedeggsandpreimplantationtoearlycleavedembryos,andnoimmunoreactivityisevidentinundifferentiatedmurineEC,ES,andEGcells.SSEA-3canbeusedtocharacterizehumanEScellsandmonitortheirdifferentiation.
ProductSpecifications
Size
100μl
Concentration
0.5mg/ml
Clone
MC-631
Isotype
RatIgM,κ
Immunogen
4-8cellstagemouseembryos
Reactivity
Mouse,Human
Preparation
Thisantibodywaspurifiedbyaffinitychromatography.
Formulation
Phosphate-bufferedsolution,pH7.2,and0.09%sodiumazide
StorageandStABIlity
Storeat4°Cprotectedfromlight.Donotfreeze.Stablefor6monthsfromdateofreceiptwhenstoredasdirected.
QualityControl
Testedbyimmunocytochemistryandflowcytometrytoensureproductquality.
RecommendedUsage
Thesuggesteduseofthisantibodyisa1:100dilutionforimmunocytochemistryand0.25µgper1x106viablecellsin100µlforflowcytometry.Forapplicationspecificprotocols,pleasereferenceProtocol:ImmunocytochemistryandProtocol:FlowCytometryonlineatwww.Stemgent.com/support/protocols.
NoticeToPurchaser
PurchaserrepresentsandwarrantsthatitwilluseStemgent®SSEA-3Antibody(Purified),Ratanti-Mouse/Humanpurelyforresearchpurposes,notfordiagnosticuse,notforresale,andnotforuseinhumansorveterinaryapplications.StemgentwillnotbeheldresponsIBLeforpatentinfringementorotherviolationsthatmayoccurwiththeuseofourproducts.
SpecificationSheets
- 09-0014SpecificationsSheet
SafetyDataSheets
- 09-0014SafetyDataSheet
Protocols
- Protocol:ImmunoflourescenceStainingofCellsforFlowCytometry
- Protocol:Immunocytochemistry
References
- Kannagi,R.,Cochran,N.A.,Ishigami,F.,Hakomori,S.,Andrews,P.W.,Knowles,B.B.,andSolter,D.(1983)Stage-specificembryonicantigens(SSEA-3and-4)areepitopesofauniqueglobo-seriesgangliosideisolatedfromhumanteratocarcinomacells.EMBOJ.2:2355-2361.
- Henderson,J.K.,Draper,J.S.,Baillie,H.S.,Fishel,S.,Thomson,J.A.,Moore,H.,andAndrews,P.W.(2002)Preimplantationhumanembryosandembryonicstemcellsshowcomparableexpressionofstage-specificembryonicantigens.StemCells20:329-337.
- Brimble,S.N.,Sherrer,E.S.,Uhl,E.W.,Wang,E.,Kelly,S.,Merrill,A.H.Jr.,Robins,A.J.,andSchulz,T.C.(2007)ThecellsurfaceglycosphingolipidsSSEA-3andSSEA-4arenotessentialforhumanESCpluripotency.StemCells25:54-62.
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