ProductOverview
Stage-specificembryonicantigen-4(SSEA-4)isexpressedinavarietyofhumancelllinesincludingembryoniccarcinoma(EC),embryonicstem(ES),andinducedpluripotentstem(iPS)cells.SSEA-4isdown-regulatedupondifferentiation,makingitawidelyusedMarkertocharacterizehumanEScellsandmonitortheirdifferentiation.Inmouse,SSEA-4isexpressedonoocytesandearlycleavage-stageembryos,butnotonECorEScells.
ProductSpecifications
Size
100μl
Concentration
0.5mg/ml
Clone
MC-813-70
Isotype
MouseIgG3
Immunogen
Humanembryoniccarcinomacellline2102Ep
Reactivity
Human
Preparation
Thisantibodywaspurifiedbyaffinitychromatography.
Formulation
Phosphate-bufferedsolution,pH7.2,and0.09%sodiumazide
StorageandStABIlity
Storeundilutedat4°Candprotectedfromlight.Donotfreeze.Stablefor6monthsfromdateofreceiptwhenstoredasdirected.
QualityControl
Testedbyimmunocytochemistryandflowcytometrytoensureproductquality.
RecommendedUsage
Thesuggesteduseofthisantibodyisa1:100dilutionforimmunocytochemistryand0.25µgper1x106viablecellsin100µlforflowcytometry.Forapplicationspecificprotocols,pleasereferenceProtocol:ImmunocytochemistryandProtocol:FlowCytometryonlineatwww.Stemgent.com/support/protocols.
NoticeToPurchaser
PurchaserrepresentsandwarrantsthatitwilluseStemgent®SSEA-4Antibody(Purified),Mouseanti-Humanpurelyforresearchpurposes,notfordiagnosticuse,notforresale,andnotforuseinhumansorveterinaryapplications.StemgentwillnotbeheldresponsIBLeforpatentinfringementorotherviolationsthatmayoccurwiththeuseofourproducts.
SpecificationSheets
- 09-0006SpecificationsSheet
SafetyDataSheets
- 09-0006SafetyDataSheet
Protocols
- Protocol:ImmunoflourescenceStainingofCellsforFlowCytometry
- Protocol:Immunocytochemistry
RelatedProducts
- mRNAReprogrammingKit(00-0071)
- microRNABoosterKit(00-0073)
References
- Brimble,S.N.,Sherrer,E.S.,Uhl,E.W.,Wang,E.,Kelly,S.,Merrill,A.H.Jr.,Robins,A.J.,andSchulz,T.C.(2007)ThecellsurfaceglycosphingolipidsSSEA-3andSSEA-4arenotessentialforhumanESCpluripotency.StemCells25:54-62.
- Kannagi,R.,Cochran,N.A.,Ishigami,F.,Hakomori,S.,Andrews,P.W.,Knowles,B.B.,andSolter,D.(1983)Stage-specificembryonicantigens(SSEA-3and-4)areepitopesofauniqueglobo-seriesgangliosideisolatedfromhumanteratocarcinomacells.EMBOJ.2:2355-2361.
- Takahashi,K.,TanabeK.,Ohnuki,M.,Narita,M.,IchisakaT.,TomodaK.andYamanaka,S.(2007)Inductionofpluripotentstemcellsfromadulthumanfibroblastsbydefinedfactors.Cell131:861-872.
- Henderson,J.K.,Draper,J.S.,Baillie,H.S.,Fishel,S.,Thomson,J.A.,Moore,H.,andAndrews,P.W.(2002)Preimplantationhumanembryosandembryonicstemcellsshowcomparableexpressionofstage-specificembryonicantigens.StemCells20:329-337.
- Battula,V.L.,Bareiss,P.M.,Treml,S.,Conrad,S.,Albert,I.,Hojak,S.,Abele,H.,Schewe,B.,Just,L.,Skutella,T.,andBuhring,H.J.(2007)HumanplacentaandbonemarrowderivedMSCculturedinserum-free,b-FGF-containingmediumexpresscellsurfacefrizzled-9andSSEA-4andgiverisetomultilineagedifferentiation.Differentiation75:279-291.
AdditionalPublications
- MoriE;FujikuraJ;NoguchiM;NakaoK;MatsubaraM;SoneM;TauraD;KusakabeT;EviharaK;TanakaT;HosadaK;TakahashiK;AsakaI;InagakiN;NakaoK."ImpairedADIpogeniccapacityininducedpluripotentstemcellsfromlipodystrophicpatientswithBSCL2mutations."Metabolism65:543(2016)
- PoleganovMA;EminliS;BeissertT;HerzS;MoonJ-I;GoldmannJ;BeyerA;HeckR;BurkhartI;RoldanDB;TureciO;YiK;HamiltonB;SahinU."Efficientreprogrammingofhumanfibroblastsandblood-derivedendothelialProgenitorcellsusingnonmodifiedRNAforreprogrammingandimmuneevasion."HumanGeneTherapy26:751(2015)
- Nagy,K.,Sung,H-K.,Zhang,P.,Laflamme,S.,Vincent,P,Agha-Mohammadi,S.,Woltjen,K.,Monetti,C.,Michael,I.P.,Smith,L.C.,Nagy,A.InducedPluripotentStemCellLinesDerivefromEquineFibroblasts.StemCellReviewsandReports7:693(2011).
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