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Stemgent/StainAlive Mouse IgM-kappa Isotype Control/09-0072/100 μl
ProductOverview
StainAliveMouseIgM,κIsotypeControl(DyLight488)isusedasanegativecontrolantibodyforstainingembryonicstem(ES)andinducedpluripotentstem(iPS)cellsinliveculture.Foruseonlivecells,thisantibodyhasbeenspecificallyformulatedwithlowendotoxinandnosodiumazide.EScellmorphology,proliferation,andtheexpressionofpluripotencyMarkersdoesnotchangeafterstainingwiththisantibody.
ProductSpecifications
Size
100μl
Concentration
0.5mg/ml
Clone
MM-30
Isotype
MouseIgM,κ
Immunogen
Trinitrophenol+KLH
Preparation
ThisantibodywaspurifiedbyaffinitychromatographyandconjugatedwithDyLight488underoptimalconditions.Thesolutionisfreeofunconjugateddyeandunlabeledantibody.0.2μmfiltersterilized.Formulationisfreefrombacteria,fungi,mycoplasma,andmousevirus.
Formulation
Phosphate-bufferedsolution,pH7.2,containingnopreservative
StorageandStABIlity
Storeat4°Cprotectedfromlight.Donotfreeze.Handleunderasepticconditions.Stablefor6monthsfromdateofreceiptwhenstoredasdirected.
QualityControl
Testedbyimmunocytochemistrytoensureproductquality.
Endotoxinlevelislessthan0.1EU/μg(0.01ng/μg)oftheproteinasdeterminedbytheLALassay.
RecommendedUsage
Thisantibodyisusedasanisotypecontrolinimmunofluorescentstaining.Useatconcentrationscomparabletothoseofthespecificantibodyofinterest.Foranapplicationspecificprotocol,pleasereferenceProtocol:ImmunocytochemistryofLiveCellsonlineatwww.Stemgent.com/support/protocols.
SpecificationSheets
- 09-0072SpecificationsSheet
SafetyDataSheets
- 09-0072SafetyDataSheet
Protocols
- GeneralProtocolforICCStainingofLiveCells
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