ProductOverview
TheTRA-1-60antibodyreactswithapluripotent,stemcellspecificantigenexpressedonundifferentiatedhumanembryonicstem(ES)cells,embryonalcarcinoma(EC)cells,andembryonicgerm(EG)cells.TheexpressionofTRA-1-60onhumanEScellsisdown-regulatedupondifferentiation.TheTRA-1-60antibodyrecognizesaneuraminidase-resistantcarbohydrateepitopeexpressedonpodocalyxin,amemberoftheCD34-relatedfamilyofsialomucins.Podocalyxinisatransmembraneglycoprotein,whichhasbeenimplicatedinthedevelopmentofaggressivenessinavarietyofcancers,includingbreastandprostatecancer.
Thisantibodyisidealforlive-stainingcells,becauseithasbeenspecificallyformulatedwithlowendotoxinandnosodiumazide.Thisallowsin-processmonitoringofreprogrammingexperimentswithoutsacrificingcells.EScellmorphology,proliferation,andexpressionofpluripotencyMarkersdoesnotchangeafterstainingwithStainAliveTRA-1-60antibody.
ProductSpecifications
Size
100μl
Concentration
0.5mg/ml
Clone
TRA-1-60
Isotype
MouseIgM,κ
Immunogen
Humanembryonalcarcinoma(EC)cellline2102Ep
Reactivity
Human
Preparation
ThisantibodywaspurifiedbyaffinitychromatographyandconjugatedwithDyLight488underoptimalconditions.Thesolutionisfreeofunconjugateddyeandunlabeledantibody.0.2μmfiltersterilized.Formulationisfreefrombacteria,fungi,mycoplasma,andmousevirus.
Formulation
Phosphate-bufferedsolution,pH7.2,containingnopreservative
StorageandStABIlity
Storeat4°Cprotectedfromlight.Donotfreeze.Handleunderasepticconditions.Stablefor6monthsfromdateofreceiptwhenstoredasdirected.
QualityControl
Testedbyimmunocytochemistrytoensureproductquality.
Endotoxinlevelislessthan0.1EU/μg(0.01ng/μg)oftheproteinasdeterminedbytheLALassay.
RecommendedUsage
Thesuggesteduseofthisantibodyisa1:100dilutionforimmunocytochemistry.Foranapplicationspecificprotocol,pleasereferenceProtocol:ImmunocytochemistryofLiveCellsonlineatwww.Stemgent.com/support/protocols.
SpecificationSheets
- 09-0068SpecificationsSheet
SafetyDataSheets
- 09-0068SafetyDataSheet
Protocols
- GeneralProtocolforICCStainingofLiveCells
RelatedProducts
- mRNAReprogrammingKit(00-0071)
- microRNABoosterKit(00-0073)
References
- Takahashi,K.,TanabeK.,Ohnuki,M.,Narita,M.,IchisakaT.,TomodaK.andYamanaka,S.(2007)Inductionofpluripotentstemcellsfromadulthumanfibroblastsbydefinedfactors.Cell131:861-872.
- Yu,J.,Vodyanik,M.A.,Smuga-Otto,K.,Antosiewicz-Bourget,J.,Frane,J.L.,Tian,S.,Nie,J.,Jonsdottir,G.A.,Ruotti,V.,Stewart,R.,Slukvin,I.I.,andThomson,J.A.(2007)Inducedpluripotentstemcelllinesderivedfromhumansomaticcells.Science318:1917-1920.
- Schopperle,W.M.,andDeWolf,W.C.(2007)TheTRA-1-60andTRA-1-81humanpluripotentstemcellmarkersareexpressedonpodocalyxininembryonalcarcinoma.StemCells25:723-730.
- Chin,A.C.,Fong,W.J.,Goh,L.T.,Philp,R.,Oh,S.K.,andChoo,A.B.(2007)Identificationofproteinsfromfeederconditionedmediumthatsupporthumanembryonicstemcells.JBiotechnol.130:320-328.
- Badcock,G.,Pigott,C.,Goepel,J.,andAndrews,P.W.(1999)ThehumanembryonalcarcinomamarkerantigenTRA-1-60isasialylatedkeratansulfateproteoglycan.CancerRes.59:4715-4719.
- Andrews,P.W.,Banting,G.,Damjanov,I.,Arnaud,D.,andAvner,P.(1984)Threemonoclonalantibodiesdefiningdistinctdifferentiationantigensassociatedwithdifferenthighmolecularweightpolypeptidesonthesurfaceofhumanembryonalcarcinomacells.Hybridoma3:347-361.
AdditionalPublications
- SridharA;OhlemacherSK;LangerKB;MeyerJS."RobustdifferentiationofmRNA-reprogrammedhumaninducedpluripotentstemcellstoardaretinallineage."StemCellTranslationalMedicine5:10(2016)
- OhlemacherSK;SridharA;XiaoY;HochstetlerAE;SarfaraziM;CumminsTR;MeyerJS."Stepwisedifferentiationofretinalganglioncellsfromhumanpluripotentstemcellsenablesanalysisofglaucomatousneurodegeneration."StemCellsdoi:10.1002/stem.2356(2016)
- PoleganovMA;EminliS;BeissertT;HerzS;MoonJ-I;GoldmannJ;BeyerA;HeckR;BurkhartI;RoldanDB;TureciO;YiK;HamiltonB;SahinU."Efficientreprogrammingofhumanfibroblastsandblood-derivedendothelialProgenitorcellsusingnonmodifiedRNAforreprogrammingandimmuneevasion."HumanGeneTherapy26:751(2015)
- BriggsSF;DominguezAA;ChavezSL;ReijoPeraRA."XISTinhumanpreimplantationembryosandiPSCs."StemCells33:1771(2015)
- WangJ;HuangV;YeL;BarcenaA;LinG;LueTF;LiL-C."IdentificationofsmallactivatingRNAsthatenhanceendogenousOCT4expressioninhumanmesenchymalstemcells."StemCellsDevelop24:345(2015)
- HaraguchiY;MatsuuraK;ShimizuT;YamatoM;OkanoT."SimplesUSPensionculturesystemofhumaniPScellsmaintiantingthepluripotencyforcardiaccellsheetengineering."JTissueEngRegenMedicine9:1363(2015)
- RajasinghS;ThangavelJ;CzirokA;SamantaS."GenerationoffunctionalcardiomyocytesfromefficientlygeneratedhumaniPSCsandanovelmethodformeasuringcontractility."PLoSone(2015)
- TanH-K;TohC-XDlMaD;YangB;LiuTM;LuJ;WongC-W;TanT-K;LiH;SYnC;TanE-L;LimB;LimY-P;CookSA;LohY-H."Humanfinger-prickinducedpluripotentstemcellsfacilitatethedevelopmentofstemcellbanking."StemCellsTransMedicine3:586(2014)
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