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Stemgent/TRA-1-81 Antibody (DyLight 488) | StainAlive/09-0069/100 μl
ProductOverview
TheTRA-1-81monoclonalantibodyreactswithapluripotent-stem-cell-specificantigenexpresseduponthesurfaceofhumanembryonicstem(ES)cells,embryonalcarcinoma(EC)cells,andembryonicgerm(EG)cells.TheexpressionofTRA-1-81antigenisstage-specific;ashumanECandEScellsundergodifferentiation,expressionofTRA-1-81antigenislost,makingitawidelyusedMarkertocharacterizehumanEScellsandtomonitortheirdifferentiation.TRA-1-81antibodyrecognizesacarbohydrateepitopeexpressedonpodocalyxin,asialoglycoproteinstructurallyrelatedtoCD34.Podocalyxinisatransmembraneglycoproteinwhichhasbeenimplicatedinthedevelopmentofaggressivenessinavarietyofcancers,includingbreastandprostatecancer.Foruseonlivecells,thisantibodyhasbeenspecificallyformulatedwithlowendotoxinandnosodiumazide.EScellmorphology,proliferation,andexpressionofpluripotencymarkersdoesnotchangeafterstainingwiththisantibody.
ProductSpecifications
Size
100μl
Concentration
0.5mg/ml
Clone
TRA-1-81
Isotype
MouseIgM,κ
Immunogen
Humanembryonalcarcinomacellline2102Ep
Reactivity
Human
Preparation
ThisantibodywaspurifiedbyaffinitychromatographyandconjugatedwithDyLight488underoptimalconditions.Thesolutionisfreeofunconjugateddyeandunlabeledantibody.0.2μmfiltersterilized.Formulationisfreefrombacteria,fungi,mycoplasma,andmousevirus.
Formulation
Phosphate-bufferedsolution,pH7.2,containingnopreservative
StorageandStABIlity
Storeat4°Cprotectedfromlight.Donotfreeze.Handleunderasepticconditions.Stablefor6monthsfromdateofreceiptwhenstoredasdirected.
QualityControl
Testedbyimmunocytochemistrytoensureproductquality.
Endotoxinlevelislessthan0.1EU/μg(0.01ng/μg)oftheproteinasdeterminedbytheLALassay.
RecommendedUsage
Thesuggesteduseofthisantibodyisa1:100dilutionforimmunocytochemistry.Foranapplicationspecificprotocol,pleasereferenceProtocol:ImmunocytochemistryofLiveCellsonlineatwww.Stemgent.com/support/protocols.
SpecificationSheets
- 09-0069SpecificationsSheet
SafetyDataSheets
- 09-0069SafetyDataSheet
Protocols
- GeneralProtocolforICCStainingofLiveCells
RelatedProducts
- mRNAReprogrammingKit(00-0071)
- microRNABoosterKit(00-0073)
References
- Andrews,P.W.,Banting,G.,Damjanov,I.,Arnaud,D.,andAvner,P.(1984)Threemonoclonalantibodiesdefiningdistinctdifferentiationantigensassociatedwithdifferenthighmolecularweightpolypeptidesonthesurfaceofhumanembryonalcarcinomacells.Hybridoma3:347-361.
- Yu,J.,Vodyanik,M.A.,Smuga-Otto,K.,Antosiewicz-Bourget,J.,Frane,J.L.,Tian,S.,Nie,J.,Jonsdottir,G.A.,Ruotti,V.,Stewart,R.,Slukvin,I.I.,andThomson,J.A.(2007)Inducedpluripotentstemcelllinesderivedfromhumansomaticcells.Science318:1917-1920.
- Henderson,J.K.,Draper,J.S.,Baillie,H.S.,Fishel,S.,Thomson,J.A.,Moore,H.,andAndrews,P.W.(2002)Preimplantationhumanembryosandembryonicstemcellsshowcomparableexpressionofstage-specificembryonicantigens.StemCells20:329-337.
- Schopperle,W.M.,andDeWolf,W.C.(2007)TheTRA-1-60andTRA-1-81humanpluripotentstemcellmarkersareexpressedonpodocalyxininembryonalcarcinoma.StemCells25:723-730.
AdditionalPublications
- PoleganovMA;EminliS;BeissertT;HerzS;MoonJ-I;GoldmannJ;BeyerA;HeckR;BurkhartI;RoldanDB;TureciO;YiK;HamiltonB;SahinU."Efficientreprogrammingofhumanfibroblastsandblood-derivedendothelialProgenitorcellsusingnonmodifiedRNAforreprogrammingandimmuneevasion."HumanGeneTherapy26:751(2015)
- HaraguchiY;MatsuuraK;ShimizuT;YamatoM;OkanoT."SimplesUSPensionculturesystemofhumaniPScellsmaintiantingthepluripotencyforcardiaccellsheetengineering."JTissueEngRegenMedicine9:1363(2015)
- Mack,A.,Kroboth,S.,Rajesh,D.,Wang,W.B.GenerationofInducedPluripotentStemCellsfromCD34+CellsacrossBloodDrawnfromMultipleDonorswithNon-IntegratingEpisomalVectors.PLoSOne;6(11):e27956.(2011)
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