ProductOverview
TheTRA-1-60monoclonalantibodyreactswithapluripotent-stem-cell-specificantigenexpressedonundifferentiatedhumanembryonicstem(ES)cells,embryonalcarcinoma(EC)cells,andembryonicgerm(EG)cells.TheexpressionofTRA-1-60onhumanEScellsisdown-regulatedupondifferentiation.TheTRA-1-60antibodyrecognizesaneuraminidase-resistantcarbohydrateepitopeexpressedonpodocalyxin,amemberoftheCD34-relatedfamilyofsialomucins.Podocalyxinisatransmembraneglycoprotein,whichhasbeenimplicatedinthedevelopmentofaggressivenessinavarietyofcancers,includingbreastandprostatecancer.
ProductSpecifications
Size
100μl
Concentration
0.5mg/ml
Clone
TRA-1-60
Isotype
MouseIgM
Immunogen
Humanembryonalcarcinomacellline2102Ep
Reactivity
Human
Preparation
Thisantibodywaspurifiedbyaffinitychromatography.
Formulation
Phosphate-bufferedsolution,pH7.2,and0.09%sodiumazide
StorageandStABIlity
Storeat4°Cprotectedfromlight.Donotfreeze.Stablefor6monthsfromdateofreceiptwhenstoredasdirected.
QualityControl
Testedbyimmunocytochemistryandflowcytometrytoensureproductquality.
RecommendedUsage
Thesuggesteduseofthisantibodyisa1:100dilutionforimmunocytochemistryand0.25µgper1x106viablecellsin100µlforflowcytometry.Forapplicationspecificprotocols,pleasereferenceProtocol:ImmunocytochemistryandProtocol:FlowCytometryonlineatwww.Stemgent.com/support/protocols.
NoticeToPurchaser
PurchaserrepresentsandwarrantsthatitwilluseStemgent®TRA-1-60Antibody(Purified),Mouseanti-Humanpurelyforresearchpurposes,notfordiagnosticuse,notforresale,andnotforuseinhumansorveterinaryapplications.StemgentwillnotbeheldresponsIBLeforpatentinfringementorotherviolationsthatmayoccurwiththeuseofourproducts.
SpecificationSheets
- 09-0010SpecificationsSheet
SafetyDataSheets
- 09-0010SafetyDataSheet
Protocols
- Protocol:ImmunoflourescenceStainingofCellsforFlowCytometry
- Protocol:Immunocytochemistry
RelatedProducts
- mRNAReprogrammingKit(00-0071)
- microRNABoosterKit(00-0073)
References
- Takahashi,K.,TanabeK.,Ohnuki,M.,Narita,M.,IchisakaT.,TomodaK.andYamanaka,S.(2007)Inductionofpluripotentstemcellsfromadulthumanfibroblastsbydefinedfactors.Cell131:861-872.
- Yu,J.,Vodyanik,M.A.,Smuga-Otto,K.,Antosiewicz-Bourget,J.,Frane,J.L.,Tian,S.,Nie,J.,Jonsdottir,G.A.,Ruotti,V.,Stewart,R.,Slukvin,I.I.,andThomson,J.A.(2007)Inducedpluripotentstemcelllinesderivedfromhumansomaticcells.Science318:1917-1920.
- Schopperle,W.M.,andDeWolf,W.C.(2007)TheTRA-1-60andTRA-1-81humanpluripotentstemcellMarkersareexpressedonpodocalyxininembryonalcarcinoma.StemCells25:723-730.
- Chin,A.C.,Fong,W.J.,Goh,L.T.,Philp,R.,Oh,S.K.,andChoo,A.B.(2007)Identificationofproteinsfromfeederconditionedmediumthatsupporthumanembryonicstemcells.JBiotechnol.130:320-328.
- Badcock,G.,Pigott,C.,Goepel,J.,andAndrews,P.W.(1999)ThehumanembryonalcarcinomamarkerantigenTRA-1-60isasialylatedkeratansulfateproteoglycan.CancerRes.59:4715-4719.
- Andrews,P.W.,Goodfellow,P.N.,Shevinsky,L.,Bronson,D.L.,andKnowles,B.B.(1982)Cellsurfaceantigensofaclonalhumanembryoniccarcinomacellline:morphologicalandantigenicdifferentiationinculture.Int.J.Cancer29:523-531.
AdditionalPublications
- MoriE;FujikuraJ;NoguchiM;NakaoK;MatsubaraM;SoneM;TauraD;KusakabeT;EviharaK;TanakaT;HosadaK;TakahashiK;AsakaI;InagakiN;NakaoK."ImpairedADIpogeniccapacityininducedpluripotentstemcellsfromlipodystrophicpatientswithBSCL2mutations."Metabolism65:543(2016)
- PoleganovMA;EminliS;BeissertT;HerzS;MoonJ-I;GoldmannJ;BeyerA;HeckR;BurkhartI;RoldanDB;TureciO;YiK;HamiltonB;SahinU."Efficientreprogrammingofhumanfibroblastsandblood-derivedendothelialProgenitorcellsusingnonmodifiedRNAforreprogrammingandimmuneevasion."HumanGeneTherapy26:751(2015)
- PaullD;SevillaA;ZhouH;HahnAK;KimH;NapolitanoC;TsankovA;ShangL;KrumholzK;JagaseesanP;WoodardCM;SunB;VilbouxT;ZimmerM;ForeroE;MoroziewiczDN;MartinezH;MalicdanMCV;WeissKA;VensandLB;DusenberryCR;PolusH;SyKTL;KahlerDJ;GahlWA;SolomonSL;ChangS;MeissnerA;EgganK;NoggleSA."Automated,high-throughputderivation,characterizationanddifferentiationofinducedpluripotentstemcells."NaureMethods12:885(2015)
- Nagy,K.,Sung,H-K.,Zhang,P.,Laflamme,S.,Vincent,P,Agha-Mohammadi,S.,Woltjen,K.,Monetti,C.,Michael,I.P.,Smith,L.C.,Nagy,A.InducedPluripotentStemCellLinesDerivefromEquineFibroblasts.StemCellReviewsandReports7:693(2011).
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