ProductOverview
TheTRA-1-81monoclonalantibodyreactswithapluripotent-stem-cell-specificantigenexpresseduponthesurfaceofhumanembryonicstem(ES)cells,embyronalcarcinoma(EC)cells,andembryonicgerm(EG)cells.TheexpressionofTRA-1-81antigenisstage-specific;ashumanECandEScellsundergodifferentiation,expressionofTRA-1-81antigenislost,makingitawidelyusedMarkertocharacterizehumanEScellsandtomonitortheirdifferentiation.TRA-1-81antibodyrecognizesacarbohydrateepitopeexpressedonpodocalyxin,asialoglycoproteinstructurallyrelatedtoCD34.Podocalyxinisatransmembraneglycoproteinwhichhasbeenimplicatedinthedevelopmentofaggressivenessinavarietyofcancers,includingbreastandprostatecancer.
ProductSpecifications
Size
100μl
Concentration
0.5mg/ml
Clone
Tra-1-81
Isotype
MouseIgM,κ
Immunogen
Humanembryonalcarcinomacellline2102Ep
Reactivity
Human
Preparation
Thisantibodywaspurifiedbyaffinitychromatography.
Formulation
Phosphate-bufferedsolution,pH7.2,and0.09%sodiumazide
StorageandStABIlity
Storeat4˚Cprotectedfromlight.Donotfreeze.Stablefor6monthsfromdateofreceiptwhenstoredasdirected.
QualityControl
Testedbyimmunocytochemistryandflowcytometrytoensureproductquality.
RecommendedUsage
Thesuggesteduseofthisantibodyisa1:100dilutionforimmunocytochemistryand0.25µgper1x106viablecellsin100µlforflowcytometry.Forapplicationspecificprotocols,pleasereferenceProtocol:ImmunocytochemistryandProtocol:FlowCytometryonlineatwww.Stemgent.com/support/protocols.
SpecificationSheets
- 09-0011SpecificationsSheet
SafetyDataSheets
- 09-0011SafetyDataSheet
Protocols
- Protocol:ImmunoflourescenceStainingofCellsforFlowCytometry
- Protocol:Immunocytochemistry
RelatedProducts
- mRNAReprogrammingKit(00-0071)
- microRNABoosterKit(00-0073)
References
- Andrews,P.W.,Banting,G.,Damjanov,I.,Arnaud,D.,andAvner,P.(1984)Threemonoclonalantibodiesdefiningdistinctdifferentiationantigensassociatedwithdifferenthighmolecularweightpolypeptidesonthesurfaceofhumanembryonalcarcinomacells.Hybridoma3:347-361.
- Yu,J.,Vodyanik,M.A.,Smuga-Otto,K.,Antosiewicz-Bourget,J.,Frane,J.L.,Tian,S.,Nie,J.,Jonsdottir,G.A.,Ruotti,V.,Stewart,R.,Slukvin,I.I.,andThomson,J.A.(2007)Inducedpluripotentstemcelllinesderivedfromhumansomaticcells.Science318:1917-1920.
- Henderson,J.K.,Draper,J.S.,Baillie,H.S.,Fishel,S.,Thomson,J.A.,Moore,H.,andAndrews,P.W.(2002)Preimplantationhumanembryosandembryonicstemcellsshowcomparableexpressionofstage-specificembryonicantigens.StemCells20:329-337.
- Schopperle,W.M.,andDeWolf,W.C.(2007)TheTRA-1-60andTRA-1-81humanpluripotentstemcellmarkersareexpressedonpodocalyxininembryonalcarcinoma.StemCells25:723-730.
AdditionalPublications
- PoleganovMA;EminliS;BeissertT;HerzS;MoonJ-I;GoldmannJ;BeyerA;HeckR;BurkhartI;RoldanDB;TureciO;YiK;HamiltonB;SahinU."Efficientreprogrammingofhumanfibroblastsandblood-derivedendothelialProgenitorcellsusingnonmodifiedRNAforreprogrammingandimmuneevasion."HumanGeneTherapy26:751(2015)
- PaullD;SevillaA;ZhouH;HahnAK;KimH;NapolitanoC;TsankovA;ShangL;KrumholzK;JagaseesanP;WoodardCM;SunB;VilbouxT;ZimmerM;ForeroE;MoroziewiczDN;MartinezH;MalicdanMCV;WeissKA;VensandLB;DusenberryCR;PolusH;SyKTL;KahlerDJ;GahlWA;SolomonSL;ChangS;MeissnerA;EgganK;NoggleSA."Automated,high-throughputderivation,characterizationanddifferentiationofinducedpluripotentstemcells."NaureMethods12:885(2015)
- Wang,Y-C.,Nakagawa,M.,Garitaonandia,I.,Slavin,I.,Altun,G.,Lacharite,R.M.,Nazor,K.L.,Tran,H.T.,Lynch,C.,L.,Leonardo,T.R.,Liu,Y.,Peterson,S.E.,Laurent,L.,C.,Yamanaka,S.,Loring,J.F.(2011)Specificlectinbiomarkersforisolationofhumanpluripotentstemcellsidentifiedthrougharray-basedglycomicanalysis.CellRes.21(11):1551-1563.
- Nagy,K.,Sung,H-K.,Zhang,P.,Laflamme,S.,Vincent,P,Agha-MohammADI,S.,Woltjen,K.,Monetti,C.,Michael,I.P.,Smith,L.C.,Nagy,A.InducedPluripotentStemCellLinesDerivefromEquineFibroblasts.StemCellReviewsandReports7:693(2011).
- Mack,A.,Kroboth,S.,Rajesh,D.,Wang,W.B.GenerationofInducedPluripotentStemCellsfromCD34+CellsacrossBloodDrawnfromMultipleDonorswithNon-IntegratingEpisomalVectors.PLoSOne;6(11):e27956.(2011)