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4000-520-616
当前位置: 首页 > 产品中心 > Functional_antibody > mu-001/mG-81/mG-01多能性;L
商品详细mu-001/mG-81/mG-01多能性;L
mu-001/mG-81/mG-01多能性;L
mu-001/mG-81/mG-01多能性;L
商品编号: 09-0011
品牌: Stemgent
市场价: ¥0.00
美元价: 0.00
产地: 美国(厂家直采)
公司:
产品分类: 功能性抗体
公司分类: Functional_antibody
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
09-0011_TRA181
ICCanalysisofTRA-1-81onH1humanEScellsat20Xmagnification.CellswerestainedwithTRA-1-81Antibody(Purified)ata1:100dilutionfollowedbyasecondaryDyLight488conjugatedantibody(green).DAPIstainingwasperformedtovisualizenuclei(blue).
09-0011_TRA181_Immunocytochemistry
ImmunocytochemistryanalysisofTRA-1-81onH1humanEScells.CellswerestainedwithTRA-1-81Antibody(Purified)ata1:100dilutionfollowedbyasecondaryCy™3conjugatedantibody(red).DAPIstainingwasperformedtovisualizenuclei(blue).
09-0011_FlowCytometryAnalysis_TRA-1-81H1hES
FlowcytometryofTRA-1-81onH1humanEScells.PurplehistogramrepresentsTRA-1-81Antibody(Purified)andopenhistogramrepresentsisotypecontrolatsameconcentration.

ProductOverview

TheTRA-1-81monoclonalantibodyreactswithapluripotent-stem-cell-specificantigenexpresseduponthesurfaceofhumanembryonicstem(ES)cells,embyronalcarcinoma(EC)cells,andembryonicgerm(EG)cells.TheexpressionofTRA-1-81antigenisstage-specific;ashumanECandEScellsundergodifferentiation,expressionofTRA-1-81antigenislost,makingitawidelyusedMarkertocharacterizehumanEScellsandtomonitortheirdifferentiation.TRA-1-81antibodyrecognizesacarbohydrateepitopeexpressedonpodocalyxin,asialoglycoproteinstructurallyrelatedtoCD34.Podocalyxinisatransmembraneglycoproteinwhichhasbeenimplicatedinthedevelopmentofaggressivenessinavarietyofcancers,includingbreastandprostatecancer.


ProductSpecifications

Size

100μl

Concentration

0.5mg/ml

Clone

Tra-1-81

Isotype

MouseIgM,κ

Immunogen

Humanembryonalcarcinomacellline2102Ep

Reactivity

Human

Preparation

Thisantibodywaspurifiedbyaffinitychromatography.

Formulation

Phosphate-bufferedsolution,pH7.2,and0.09%sodiumazide

StorageandStABIlity

Storeat4˚Cprotectedfromlight.Donotfreeze.Stablefor6monthsfromdateofreceiptwhenstoredasdirected.

QualityControl

Testedbyimmunocytochemistryandflowcytometrytoensureproductquality.

RecommendedUsage

Thesuggesteduseofthisantibodyisa1:100dilutionforimmunocytochemistryand0.25µgper1x106viablecellsin100µlforflowcytometry.Forapplicationspecificprotocols,pleasereferenceProtocol:ImmunocytochemistryandProtocol:FlowCytometryonlineatwww.Stemgent.com/support/protocols.


SpecificationSheets

  • 09-0011SpecificationsSheet

SafetyDataSheets

  • 09-0011SafetyDataSheet

Protocols

  • Protocol:ImmunoflourescenceStainingofCellsforFlowCytometry
  • Protocol:Immunocytochemistry

RelatedProducts

  • mRNAReprogrammingKit(00-0071)
  • microRNABoosterKit(00-0073)

References

  1. Andrews,P.W.,Banting,G.,Damjanov,I.,Arnaud,D.,andAvner,P.(1984)Threemonoclonalantibodiesdefiningdistinctdifferentiationantigensassociatedwithdifferenthighmolecularweightpolypeptidesonthesurfaceofhumanembryonalcarcinomacells.Hybridoma3:347-361.
  2. Yu,J.,Vodyanik,M.A.,Smuga-Otto,K.,Antosiewicz-Bourget,J.,Frane,J.L.,Tian,S.,Nie,J.,Jonsdottir,G.A.,Ruotti,V.,Stewart,R.,Slukvin,I.I.,andThomson,J.A.(2007)Inducedpluripotentstemcelllinesderivedfromhumansomaticcells.Science318:1917-1920.
  3. Henderson,J.K.,Draper,J.S.,Baillie,H.S.,Fishel,S.,Thomson,J.A.,Moore,H.,andAndrews,P.W.(2002)Preimplantationhumanembryosandembryonicstemcellsshowcomparableexpressionofstage-specificembryonicantigens.StemCells20:329-337.
  4. Schopperle,W.M.,andDeWolf,W.C.(2007)TheTRA-1-60andTRA-1-81humanpluripotentstemcellmarkersareexpressedonpodocalyxininembryonalcarcinoma.StemCells25:723-730.

AdditionalPublications

  • PoleganovMA;EminliS;BeissertT;HerzS;MoonJ-I;GoldmannJ;BeyerA;HeckR;BurkhartI;RoldanDB;TureciO;YiK;HamiltonB;SahinU."Efficientreprogrammingofhumanfibroblastsandblood-derivedendothelialProgenitorcellsusingnonmodifiedRNAforreprogrammingandimmuneevasion."HumanGeneTherapy26:751(2015)
  • PaullD;SevillaA;ZhouH;HahnAK;KimH;NapolitanoC;TsankovA;ShangL;KrumholzK;JagaseesanP;WoodardCM;SunB;VilbouxT;ZimmerM;ForeroE;MoroziewiczDN;MartinezH;MalicdanMCV;WeissKA;VensandLB;DusenberryCR;PolusH;SyKTL;KahlerDJ;GahlWA;SolomonSL;ChangS;MeissnerA;EgganK;NoggleSA."Automated,high-throughputderivation,characterizationanddifferentiationofinducedpluripotentstemcells."NaureMethods12:885(2015)
  • Wang,Y-C.,Nakagawa,M.,Garitaonandia,I.,Slavin,I.,Altun,G.,Lacharite,R.M.,Nazor,K.L.,Tran,H.T.,Lynch,C.,L.,Leonardo,T.R.,Liu,Y.,Peterson,S.E.,Laurent,L.,C.,Yamanaka,S.,Loring,J.F.(2011)Specificlectinbiomarkersforisolationofhumanpluripotentstemcellsidentifiedthrougharray-basedglycomicanalysis.CellRes.21(11):1551-1563.
  • Nagy,K.,Sung,H-K.,Zhang,P.,Laflamme,S.,Vincent,P,Agha-MohammADI,S.,Woltjen,K.,Monetti,C.,Michael,I.P.,Smith,L.C.,Nagy,A.InducedPluripotentStemCellLinesDerivefromEquineFibroblasts.StemCellReviewsandReports7:693(2011).
  • Mack,A.,Kroboth,S.,Rajesh,D.,Wang,W.B.GenerationofInducedPluripotentStemCellsfromCD34+CellsacrossBloodDrawnfromMultipleDonorswithNon-IntegratingEpisomalVectors.PLoSOne;6(11):e27956.(2011)
品牌介绍
Stemgent是一个美国注册的公司。它的背景是为生物科学家提供干细胞研究的试剂与试剂盒。的分裂,也可以变成或分化成体内各种组织器官的细胞。胚胎干细胞可以形成完整的胚胎或个体。这就是个体克隆。壮大Stemgent的宗旨是提供各种试剂与试剂盒,配备生物科研人员新的研究工具与手段。Stemgent 通过提供由一些世界领先的干细胞科学家开发的专有试剂和工具来推进干细胞科学。Stemgents 产品针对干细胞进行了专门优化和筛选,包括用于多能性、自我更新和分化的小分子、病毒传递的转录因子、基质、细胞系、细胞因子、抗体、转染试剂等。这种独特的产品组合旨在服务于研究干细胞生物学和再生医学的研究人员,以及使用源自干细胞的细胞作为工具来促进他们对主要疾病的了解的研究人员。拥有双总部波士顿和 圣地亚哥, 认证机构, Stemgent 有能力为这些主要的研究市场提供服务。来到这里是为了一个目的:帮助您推进干细胞科学。我们通过提供由一些世界领先的干细胞科学家开发并证明可重复性的专有试剂和工具来做到这一点。