ProductOverview
Neonatalhumanforeskinfibroblasts(NuFFs)aremitoticallyinactivatedfibroblastsproducedbyGlobalStemandfunctionallyvalidatedbyStemgenttosupportthegenerationofinducedpluripotentstem(iPS)cellcoloniesfromhumannewbornforeskinfibroblastsusingtheStemgentmRNAReprogrammingKit.NuFF-RQcellscanalsobeusedtocondition Pluriton™ReprogrammingMedium andimprovetheproductivityofiPSgenerationinfeeder-freeandextendedfeeder-basedreprogrammingcultures.
ProductSpecifications
Size
4-5x106cellspervial
Species
Homosapien
Source
Normal,NeonatalForeskinFibroblasts,donor12
StorageandStABIlity
Storeinvaporphaseliquidnitrogen.Stableforaminimumof6monthsfromdateofreceiptwhenstoredasdirected.
QualityControl
Cellstestednegativeformycoplasma,bacteria,fungi,andsporecontamination.WhenthawedandculturedaccordingtoStemgentprotocolsNuFF-RQcellsareguaranteedtobegreaterthan80%viableuponthaw.
Propagation
Foruseasafeederlayerand/orconditioningPluriton™ReprogrammingMedium,pleasereferenceUserManual:StemgentmRNAReprogrammingSystemand/orProtocol: GeneratingNuFF-ConditionedPluritonMedium.
NoticetoPurchaser
PurchaserrepresentsandwarrantsthatitwilluseNuFF-RQIRR cellspurelyforresearchpurposes,notfordiagnosticuse,notforresale,andnotforuseinhumansorveterinaryapplications.StemgentwillnotbeheldresponsIBLeforpatentinfringementorotherviolationsthatmayoccurwiththeuseofourproducts.
RestrictedUse
ForrestrictionsrelatedtotheuseofthisproductpleaserefertotheaccompanyingRestrictedUseLicence.
SpecificationSheets
- PS-CL-GSC3002G
SafetyDataSheets
- GSC-3002GSafetyDataSheet
ProductSheets
- ProductSheet_RNAReprogramming
RelatedProducts
- microRNABoosterKit(00-0073)
- mRNAReprogrammingKit(00-0071)
- ReprogrammingMedium(00-0070)
References
- Warren,L.,Manos,P.D.,Ahfeldt,T.,Loh,Y.H.,Li,H.,Lau,F.,Ebina,W.,Mandal,P.K.,Smith,Z.D.,Meissner,A.,Daley,D.Q.,Brack,A.S.,Collins,J.J.,Cowan,C.,Schlaeger,T.M.,andRossi,D.J.(2010)HighlyefficientreprogrammingtopluripotencyanddirecteddifferentiationofhumancellswithsyntheticmodifiedmRNA.CellStemCell7:618-630.
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