ProductOverview
StemfactorActivinA isacrystallography-gradepreparationprovidingthehighestpurityavailable.ActivinAisbi-modalinactionhavingbeenshowntomaintainpluripotencyofstemcellsandpromotedifferentiation1,2,3,4.MaturerecombinanthumanActivinAisa~25kDadisulfide-linkedhomodimeroftwo116aminoacidresidueβAsubunits.
Stemgentconvenientlyprovides all thecomponents comprising the5i/L/Amediasupplmentnecessarytosupportnaivepluripotentstemcellapplications.
5i(inhibitors):
- Stemolecule™PD0325901(04-0006),
- Stemolecule™Y27632(04-0012),
- Stemolecule™WH-4-023(04-0079),
- Stemolecule™SB590885(04-0080),
- Stemolecule™IM-12(04-0081)oralternatively,
Stemolecule™CHIR99021(04-0004)
L:Stemfactor™LIF,HumanRecombinant(03-0016),and
A:Stemfactor™ActivinA,HumanRecombinant(03-0001)
ProductSpecifications
Size
5μg
Source
E.Coli
Formulation
Lyophilizedfrom20%acetonitrile
AminoAcidSequence
GLECDGKVNICCKKQFFVSFKDIGWNDWIIAPSGYHANYCEGECPSHIAGTSGSSLSFHSTVINHYRMRGHSPFANLKSCCVPTKLRPMSMLYYDDGQNIIKKDIQNMIVEECGCS
UniprotAccessionNumber
P08476,residues311-426
Purity
Greaterthan98%bySDS-PAGEanalysis
EndotoxinLevel
Lessthan 1.0EU/μgofActivinAasdeterminedbytheLALmethod.
BIOLOGicActivity
TheED50islessthan 6ng/mlasdeterminedbyitsABIlitytoinhibittheproliferationofmouseMPC-11cells.
StorageandStability
ActivinAisshippedatroomtemperature.LyophilizedActivinAisstableforupto6monthsfromdateofreceiptwhenstoredat-20°Cto-80°C. ReconstitutedActivinA,atconcentrationsgreaterthan0.1mg/ml,isstableforupto3monthswhenstoredat-20°Candfor1yearwhenstoredat-80°C
Reconstitution
CentrifugebrieflyandreconstituteActivinAin10mMHClbeforedilutionincellculturemedium.Avoidfreeze-thawcyclesasitcanresultinlossofactivity.
SpecificationSheets
- 03-0001SpecificationsSheet
SafetyDataSheets
- 03-0001SafetyDataSheet
References
- Beattie,G.M.,Lopez,A.D.,Bucay,N.,Hinton,A.,Firpo,M.T.,King,C.C.,andHayek,A.(2005)ActivinAmaintainspluripotencyofhumanembryonicstemcellsintheabsenceoffeederlayers.StemCells23:489-495.
- Vallier,L.,Alexander,M.,andPedersen,R.A.(2005)Activin/NodalandFGFpathwayscorporatetomaintainpluripotencyofhumanembryonicstemcells.J.CellSci.118:4495-4509.
- Sulzbacher,S.,Schroeder,I.S.,Truong,T.T.,andWobus,A.M.(2009)ActivinA-induceddifferentiationofembryonicstemcellsintoendodermandpancreaticProgenitors-theinfluenceofdifferentiationfactorsandcultureconditions.StemCellRev.5:159-173.
- Valdimarsdottir,G.,andMummery,C.(2005)FunctionsoftheTGFbetasuperfamilyinhumanembryoniccells.APMIS113:773-789.
AdditionalPublications
- Villa-DiazLG;KimJK;LaperleA;PalecekSP;KresbachPH."Inhibitionoffocaladhesionkinasesignalingbyintegrina6b1supportshumanstemcellself-renewal."StemCellsdoi:10.1002/stem.2349(2016)
- ZhuS;RussHA;WangX;ZhangM;MaT;XuT;TangS;HebrokM;DengS."Humanpancreaticbeta-likecellsconvertedfromfibroblasts."NatureCommun7:10080(2016)
- SaxenaS;RonnRE;GuibentifC;MoraghebaR;WoodsN-B."CyclicAMPsignalingthroughEpacaxismodulateshumanhemogenicendotheliumandenhanceshematopoeiticcellgeneration."StemCellRepdoi:10.1016/j.stemcr.2016.03.006(2016)
- Mora-CastillaS;ToC;VaezeslamiS;MoreyR;SrinivasanS;DumdieJN;Cook-AndersenH;JenkinsJ;LaurentLC."Miniaturizationtechnologiesforefficientsingle-celllibrarypreparationfornext-generationsequencing."JLabAutomation2016:1(2016)
- QianX."Definingthemechanismbywhichsyntheticpolymersurfacessupporthumanpluripotentstemcellself-renewal."PhDThesis,UniversityofMichigan(2015)
- Villa-DiazLG;KimJK;LahannJ;KresbachPH."Derivationandlong-termcultureoftransfene-freehumaninducedpluripotentstemcellsonsyntheticsubstates."StemCellsTransMed3:1410(2014)
- Bukong,T.N.,Lo,T.,Szabo,G.,andDolganiuc,A.(2012)Noveldevelopmentalbiology-basedprotocolofembryonicstemcelldifferentiationtomorphologicallysoundandfunctionalyetimmaturehepatocytes.LiverInt.doi:10.1111/j.1478-3231.2011.02743.x.[Epubaheadofprint]
- Kunisada,Y.,Tsubooka-Yamazoe,N.,Shoji,M.,andHosoya,M.(2012)Smallmoleculesinduceefficientdifferentiationintoinsulin-producingcellsfromhumaninducedpluripotentstemcells.StemCellRes8:274-284.
- McCracken,K.W.,Howell,J.C.,Wells,J.M.,andSpence,J.R.(2011)Generatinghumanintestinaltissuefrompluripotentstemcellsinvitro.NatProtoc6:1920-1928.
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