ProductOverview
Reprogramming-qualifiedB18RRecombinantProtein,Carrier-free(#03-0017) isusefulforcellularreprogrammingandotherapplicationsrequiringRNA-mediatedgenedelivery. B18RproteinisaVacciniavirus-encodedreceptorwithspecificityformouse,human,rabbit,pig,ratandcowType1interferonsandhaspotentneutralizingcapABIlityactingas adecoyreceptorforType1interferons.
PreviouslyonlyavailableaspartofthemRNAReprogrammingKit(#00-0071) ormicroRNABoosterKit(#00-0073), Reprogramming-qualifiedB18RRecombinantProtein,Carrier-freeisnowavailableforindividualpurchase.
ProductSpecifications
ProductName
StemfactorTMB18RRecombinantProtein,Carrier-Free
CatalogNumber
03-0017
Quantity
50ugin100uL
Concentration
0.5mg/mL
Source
InsectcellsinfectedwithbaculoviruscontainingaminoacidsHis20–Glu351ofvacciniavirusB18RaccessionnumberD01019,withaHistag.
Formulation
B18Rissuppliedasasterileliquidin10mMphosphate,0.5MNaClwithapHof7.5
Purity
Greaterthan98%bySDS-PAGEanalysis
EndotoxinLevel
Lessthan0.5EU/mgofB18RasdeterminedbytheLALmethod
BIOLOGicalActivity
StemfactorB18RRecombinantProtein,Carrier-freebioactivityisdemonstratedbytheneutralizationofIFN-alphaorIFN-betaeffectsinanassayofIL-12productionfromCD40L-activatedmonocytes
Thebiologicalfunctionisvalidatedbydown-regulationofgeneexpressionbyqRT-PCRofTLR3,IFIT1,andOASIinresponsetoB18Rtreatmentonhumanfibroblaststolevelsequivalenttonon-transfectedcells.
TheefficiencyofmRNAreprogrammingistestedbyperformingmRNAreprogrammingwithOKSMLonneonatalforeskinfibroblastsanddemonstratinggreaterthan0.5%iPSCcolonyformation.
Sterility
TestedtobenegativeforMycoplasmasp.byRT-PCRandnegativeformicrobialcontaminationbysterilitytesting.
StorageandHandling
StemfactorB18RRecombinantProtein,Carrier-freeisshippedondryice. Forlong-termstorage,aliquotandstoreat-80degreesC.Avoidfreeze-thawcycles.
Stability
Stableforupto6monthsfromdateofreceiptwhenstoredasdirected.
Manufacturedby
References
WarrenL,ManosPD,AhfedltT,LohYH,LiH,LauF,EbinaW,MandalPK,SmithZD,MeissnerA,DaleyGQ,BrackAS,CollinsJJ,CowanC,SchlaegerTM,RossiDJ.HighlyefficientreprogrammingtopluripotencyanddirecteddifferentiationofhumancellswithsyntheticmodifiedmRNA.CellStemCell,7(5):618-30(2010).
SpecificationSheets
- 03-0017SpecifcationSheet
SafetyDataSheets
- 03-0017SafetyDataSheet