ProductOverview
BoneMorphogeneticProtein4(BMP-4)isapolypeptidebelongingtotheTGF-βproteinsuper-family.BMP-4isinvolvedinboneandcartilagedevelopment;morespecifically,intoothandlimbdevelopmentfracturerepair1.Inhumanembryonicdevelopment,BMP-4isacriticalsignalingmoleculerequiredfortheearlydifferentiationoftheembryoandestablishmentofadorsal-ventralaxis2,3.BMP-4playsanimportantroleindifferentiationofoverlyingectodermaltissue.InhibitionoftheBMP-4signalcausestheectodermtodifferentiateintotheneuralplate.Inculturedstemcells,BMP-4playsadistinctroleinmouseandhumanembryonicstem(ES)cells.BMP-4supportsLIFasapositivefactorformouseEScellself-renewal4.Incontrast,BMP-4inducesextra-embryonictrophoblastdifferentiationinhumanEScells5. StemfactorBMP-4isarecombinantproteinexpressedandpurifiedfromhuman293cellsasaglycosylatedhomodimerwithamolecularmassof34kDa.
ProductSpecifications
Size
10µg
Source
StemfactorBMP-4wasexpressedinandpurifiedfromhuman293cells.
Formulation
Lyophilizedfromsterile-filtered50mMNaOAc,pH4.5and1MNaCl.
AminoAcidSequence
SPKHHSQRARKKNKNCRRHSLYVDFSDVGWNDWIVAPPGYQAFYCHGDCPFPLADHLNSTNHAIVQTLVNSVNSSIPKACCVPTELSAISMLYLDEYDKVVLKNYQEMVVEGCGCR
UniprotAccessionNumber
P12644,residues293-408
Purity
Greaterthan95%bySDS-PAGEanalysis.
EndotoxinLevel
Lessthan1.0EU/μgofBMP-4asdeterminedbytheLALmethod.
BIOLOGicActivity
TheED50islessthan30ng/mlasdeterminedbyitsABIlitytoinducealkalinephosphataseproductionbymousechondrogenicATDC-5cells.
Sterility
TestedtobenegativeforMycoplasmasp.byPCRandmicrobialcontaminationbya sterilitytest.
StorageandStability
StemfactorBMP-4isshippedatroomtemperature.LyophilizedBMP-4isstableforupto6monthsfromdateofreceiptwhenstoredat-20°Cto-80°C.ReconstitutedBMP-4,atconcentrationsgreaterthanorequalto0.1mg/ml,isstableforupto3monthswhenstoredat-20°Candupto6monthswhenstoredat-80°C.
Reconstitution
CentrifugebrieflyandthenreconstituteBMP-4in4mMHCltoyieldastocksolutionofnolessthan0.1mg/ml.Avoidfreeze-thawcyclesasitcanresultinlossofactivity.
SpecificationSheets
- 03-0007SpecificationsSheet
SafetyDataSheets
- 03-0007SafetyDataSheet
References
- Miljkovic,N.D.,Cooper,G.M.,andMarra,K.G.(2008)Chondrogenesis,bonemorphogeneticprotein-4andmesenchymalstemcells.OsteoarthritisCartilage16:1121-1130.
- Chen,D.,Zhao,M.,andMundy,G.R.(2004)Bonemorphogeneticproteins.GrowthFactors22:233-241.
- Sadlon,T.J.,Lewis,I.D.,andD’Andrea,R.J.(2004)BMP4:itsroleindevelopmentofthehematopoieticsystemandpotentialasahematopoieticgrowthfactor.StemCells22:457-474.
- Ying,Q.L.,Nichols,J.,Chambers,I.,andSmith,A.(2003)BMPinductionofIdproteinssuppressesdifferentiationandsustainsembryonicstemcellself-renewalincollaborationwithSTAT3.Cell115:281-292.
- Xu,R.H.,Chen,X.,Li,D.S.,Li,R.,Addicks,G.C.,Glennon,C.,Zwaka,T.P.,andThompson,J.A.(2002)BMP4initiateshumanembryonicstemcelldifferentiationtotrophoblasts.NatBiotechnol.20:1261-1264.
AdditionalPublications
- SaxenaS;RonnRE;GuibentifC;MoraghebaR;WoodsN-B."CyclicAMPsignalingthroughEpacaxismodulateshumanhemogenicendotheliumandenhanceshematopoeiticcellgeneration."StemCellRepdoi:10.1016/j.stemcr.2016.03.006(2016)
- QianX."Definingthemechanismbywhichsyntheticpolymersurfacessupporthumanpluripotentstemcellself-renewal."PhDThesis,UniversityofMichigan(2015)
- ZhuS;WangH;DengS."Reprogrammingfibroblaststowardcardiomyocytes,neuralstemcellsandhepatocytesbycellactivationandsignaling-directedlineageconversion."NatureProtocols10,959-973(2015)
- Villa-DiazLG;KimJK;LahannJ;KresbachPH."Derivationandlong-termcultureoftransfene-freehumaninducedpluripotentstemcellsonsyntheticsubstates."StemCellsTransMed3:1410(2014)
- ChiangP-M;WongPC."Differentiationofanembryonicstemcelltohemogenicendotheliumbydefinedfactors:Essentialroleofbonemorphogeneticprotein4."Development138:2833-2843(2011)
- Wang,B.,Miyagoe-Suzuki,Y.,Yada,E.,Ito,N.,Nishiyama,T.,Nakamura,M.,Ono,Y.,Motohashi,N.,Segawa,M.,Masuda,S.,Takeda,S.ReprogrammingEfficiencyandQualityofInducedPluripotentStemCells(iPSCs)GeneratedfromMuscle-derivedFibroblastsofMDXMiceatDifferentAges.PLOSCurrentsMuscularDystrophy.2011Oct27.Edition1.doi:10.1371/currents.RRN1274.
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