ProductOverview
FibroblastGrowthFactor-basic(bFGF),alsoknownasFGF-2,isaheparin-bindingmemberoftheFGFsuperfamilyofmolecules.Proteinsofthisfamilyplayacentralroleduringprenataldevelopmentandpostnatalgrowthandregenerationofavarietyoftissuesbypromotingcellularproliferationanddifferentiation.Additionally,bFGFisacriticalcomponentofembryonicstemcellculturemedium,allowingcellstoremaininanundifferentiatedstateinserum-freemedium1,2.StemfactorbFGFisanapproximate17kDaproteinconsistingof155aminoacidresidues.
ProductSpecifications
Size
50μg
Source
E.coli
Formulation
Lyophilizedfrom5mMTris-HCl,pH7.5with150mMNaCl.
AminoAcidSequence
MAAGSITTLPALPEDGGSGAFPPGHFKDPKRLYCKNGGFFLRIHPDGRVDGVREKSDPHIKLQLQAEERGVVSIKGVCANRYLAMKEDGRLLASKCVTDECFFFERLESNNYNTYRSRKYTSWYVALKRTGQYKLGSKTGPGQKAILFLPMSAKS
UniprotAccessionNumber
P09038,residues1-155
Purity
Greaterthan95%bySDS-PAGEanalysis
EndotoxinLevel
Lessthan1.0EU/μgofbFGFasdeterminedbytheLALmethod.
BIOLOGicActivity
TheED50is0.1to1ng/mlasdeterminedbythedosedependentproliferationofNIH3T3cells.
Sterility
TestedtobenegativeforMycoplasmasp.byPCRandmicrobialcontaminationbya sterilitytest.
StorageandStABIlity
StemfactorbFGFisshippedatroomtemperature.LyophilizedbFGFisstableforupto6monthsfromdateofreceiptwhenstoredat-20°Cto-80°C.ReconstitutedbFGF,atconcentrationsgreaterthanorequalto0.1mg/ml,isstableforupto3monthswhenstoredat-20°Candupto6monthswhenstoredat-80°C.
Reconstitution
CentrifugebrieflyandthenreconstitutebFGFin500μlof10mMTris,pH7.6,toyieldastocksolutionof0.1mg/mlofbFGF.Avoidfreeze-thawcyclesasitcanresultinlossofactivity.
SpecificationSheets
- 03-0002SpecificationsSheet
SafetyDataSheets
- 03-0002SafetyDataSheet
ApplicationNotes
- bFGFSupportsHumanESCellSelf-Renewal
RelatedProducts
- mRNAReprogrammingKit(00-0071)
- microRNABoosterKit(00-0073)
References
- Amit,M.,Carpenter,M.K.,Inokuma,M.S.,Chiu,C.P.,Harris,C.P.,Waknitz,M.A.,Itskovitz-Eldor,J.,andThompson,J.A.(2000)Clonallyderivedhumanembryonicstemcelllinesmaintainpluripotencyandproliferativepotentialforprolongedperiodsofculture.DevBiol227:271-278.
- Xu,C.,Rosler,E.,Jiang,J.,Lebkowski,J.S.,Gold,J.D.,O’Sullivan,C.,Delavan-Boorsma,K.,Mok,M.,Bronstein,A.,andCarpenter,M.K.(2005)Basicfibroblastgrowthfactorsupportsundifferentiatedhumanembryonicstemcellgrowthwithoutconditionedmedium.StemCells23:315-323.
- Vallier,L.,Alexander,M.,andPedersen,R.A.(2005)Activin/NodalandFGFpathwayscorporatetomaintainpluripotencyofhumanembryonicstemcells.J.CellSci.118:4495-4509.
- Wang,G.,Zhang,H.,Zhao,Y.,Li,J.,Cai,J.,Wang,P.,Meng,S.,Feng,J.,Miao,C.,Ding,M.,Li,D.,andDeng,H.(2005)NogginandbFGFcooperatetomaintainthepluripotencyofhumanembryonicstemcellsintheabsenceoffeederlayers.BiochemBiophysResCommun330:934-942.
- Greber,B.,Lehrach,H.,andAdjaye,J.(2007)Fibroblastgrowthfactor2modulatestransforminggrowthfactorbetasignalinginmouseembryonicfibroblastsandhumanESCs(hESCs)tosupporthESCself-renewal.StemCells25:455-464.
AdditionalPublications
- MomcilovicO;SivapathamR;OronTR;MeyerM;MooneyS;RaoMA;ZengX."Derivation,characteriazation,andneuraldifferentiationofintegration-freeinducedpluripotentstemcelllinesfromParkinson"sdiseasepatientscfarryingSNCA,LRRK2,PARK2,andGBAmutations."PLoSONE11(5):e0154890.doi:10.1371/journal.pone.0154890(2016)
- BhutaniK;NazorKL;WilliamsR;TranH;DaiH;DzakulaZZ;ChoEH;PangAWC;RoaM;CaoH;SchorckNJ;LoringJF."Whole-genomemutationalburdenanalysisofthreepluripotencyinductionmethods."NatureCommun7:10536(2016)
- ZhuS;RussHA;WangX;ZhangM;MaT;XuT;TangS;HebrokM;DengS."Humanpancreaticbeta-likecellsconvertedfromfibroblasts."NatureCommun7:10080(2016)
- ChiangP-M;WongPC."Differentiationofanembryonicstemcelltohemogenicendotheliumbydefinedfactors:Essentialroleofbonemorphogeneticprotein4."Development138:2833-2843(2011)
- Wang,Y-C.,Nakagawa,M.,Garitaonandia,I.,Slavin,I.,Altun,G.,Lacharite,R.M.,Nazor,K.L.,Tran,H.T.,Lynch,C.,L.,Leonardo,T.R.,Liu,Y.,Peterson,S.E.,Laurent,L.,C.,Yamanaka,S.,Loring,J.F.(2011)SpecificlectinbioMarkersforisolationofhumanpluripotentstemcellsidentifiedthrougharray-basedglycomicanalysis.CellRes.21(11):1551-1563.
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