ProductOverview
RecombinantHumanLIFisalymphoidfactorthatpromoteslong-termmaintenanceofthepluripotencyofembryonicstemcellsbysuppressingspontaneousdifferentiation1,2.LeukemiaInhibitoryFactor(LIF)hasadditionalfunctionssuchascholinergicneurondifferentiation,boneandfatmetabolism,mitogenesisofcertainfactordependentcelllinesandpromotionofmegakaryocyteproductioninvivo3.HumanLIFisanapproximate20kDaproteincontaining202aminoacidresiduesandisactiveonbothhumanandmousecells.
Stemgentconvenientlyprovides all thecomponents comprising the5i/L/Amediasupplmentnecessarytosupportnaivepluripotentstemcellapplications.
5i(inhibitors):
- Stemolecule™PD0325901(04-0006),
- Stemolecule™Y27632(04-0012),
- Stemolecule™WH-4-023(04-0079),
- Stemolecule™SB590885(04-0080),
- Stemolecule™IM-12(04-0081)oralternatively,
Stemolecule™CHIR99021(04-0004)
L:Stemfactor™LIF,HumanRecombinant(03-0016),and
A:Stemfactor™ActivinA,HumanRecombinant(03-0001)
ProductSpecifications
Size
1ml
Concentration
10μg/ml(Cat.No.03-0016)
100μg/ml(Cat.No.03-0016-100)
Source
E.coli
Purity
Greaterthan99%bySDS-PAGEanalysis.
Formulation
LIFissuppliedasa0.22µmsterilefilteredliquidinPBScontaining1%w/vBSAascarrier.
EndotoxinLevel
Lessthan1EU/μgofLIFasdeterminedbytheLALmethod.
BIOLOGicActivity
HumanLIFactivityisassessedbyitsABIlitytoinducedifferentiationofM1myeloidleukemiacells.Thespecificactivityis1x106units/ml(Cat.No.03-0016)and1x107units/ml(Cat.No.03-0016-100),where50unitsisdefinedastheamountofhumanLIFrequiredtoinducedifferentiationin50%oftheM1coloniesin1mlofmedium.
StorageandStability
StoreLIFat4°C.Donotfreeze.Stableforupto6monthsfromdateofreceipt,whenstoredasdirected.
SpecificationSheets
- 03-0016SpecificationsSheet
- 03-0016-100SpecificationsSheet
SafetyDataSheets
- 03-0016and03-0016-100SafetyDataSheet
RelatedProducts
- ReproNaiveMedium(RC-HEMD008)
References
- Williams,R.L.,Hilton,D.J.,Pease,S.,Willson,T.A.,Stewart,C.L.,Gearing,D.P.,Wagner,E.F.,Metcalf,D.,Nicola,N.A.,andGough,N.M.(1988)Myeloidleukemiainhibitoryfactormaintainsthedevelopmentalpotentialofembryonicstemcells.Nature336:684-687.
- Gough,N.M.,Gearing,D.P.,King,J.A.,Willson,T.A.,Hilton,D.J.,Nicola,N.A.,andMetcalf,D.(1988)Molecularcloningandexpressionofthehumanhomologueofthemurinegeneencodingmyeloidleukemia-inhibitoryfactor.Proc.Natl.Acad.Sci.USA85:2623-2627.
- Hanna,J.,Cheng,A.W.,Saha,K.,Kim,J.,Lengner,C.J.,Soldner,F.,Cassady,J.P.,Muffat,J.,Carey,B.W.,andJaenisch,R.(2010)HumanembryonicstemcellswithbiologicalandepigeneticcharacteristicssimilartothoseofmouseESCs.Proc.Natl.Acad.Sci.USA107:9222-9227.
AdditionalPublications
- MetcalfD.(2003)Theunsolvedenigmasofleukemiainhibitoryfactor.StemCells21:5-14.
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