ProductOverview
MouseLIFisalymphoidfactorthatpromoteslong-termmaintenanceofthepluripotencyofmouseembryonicstemcellsbysuppressingspontaneousdifferentiation1,2.LeukemiaInhibitoryFactor(LIF)hasadditionalfunctionssuchascholinergicneurondifferentiation,boneandfatmetabolism,mitogenesisofcertainfactordependentcelllinesandpromotionofmegakaryocyteproductioninvivo.MouseLIFisanapproximate20kDaproteincontaining202aminoacidresidues.
ProductSpecifications
Size
1ml
Concentration
10μg/ml(Cat.No.03-0011)
100μg/ml(Cat.No.03-0011-100)
Source
E.coli
Purity
Greaterthan98%bySDS-PAGEanalysis.
Formulation
LIFissuppliedasa0.22µmsterilefilteredliquidinPBScontaining1%w/vBSAascarrier.
EndotoxinLevel
Lessthan1EU/μgofLIFasdeterminedbytheLALmethod.
BIOLOGicActivity
MouseLIFactivityisassessedbyitsABIlitytoinducedifferentiationofM1myeloidleukemiacells.Thespecificactivityis1x106units/ml(Cat.No.03-0011)and1x107units/ml(Cat.No.03-0011-100),where50unitsisdefinedastheamountofmouseLIFrequiredtoinducedifferentiationin50%oftheM1coloniesin1ml ofmedium.
RecommendedUsage
1mlofmouseLIFwiththespecificactivityof1x106units/mlissufficienttotreat1Lofembryonicstemcellculturemedia.
1mlofmouseLIFwiththespecificactivityof1x107units/mlissufficienttotreat10Lofembryonicstemcellculturemedia.
Sterility
TestedtobenegativeformycoplasmabyPCRandmicrobialcontaminationbya sterilitytest.
StorageandStability
LIFisshippedatroomtemperature.ConcentratedLIFisstableforupto6monthsfromdateofreceiptwhenstoredat4°C.PriortousediluteLIFinsteriletissueculturemedia,aliquottoaconvenientconcentration,andstoreat4°C.Avoidfreeze-thawcyclesasitcanresultinlossofactivity.
SpecificationSheets
- 03-0011SpecificationsSheet
- 03-0011-100SpecificationsSheet
SafetyDataSheets
- 03-0011and03-0011-100SafetyDataSheet
References
- Niwa,H.,Ogawa,K.,Shimosato,D.,andAdachi,K.(2009)AparallelcircuitofLIFsignallingpathwaysmaintainspluripotencyofmouseEScells.Nature460:118-122.
- Xu,J.,Wang,F.,Tang,Z.,Zhan,Y.,Zhang,J.,Yan,Q.,Xiong,Y.,Xie,X.,Wu,J.,andZhang,S.(2010)Roleofleukaemiainhibitoryfactorintheinductionofpluripotentstemcellsinmice.CellBiolInt.34:791-797.
- Fukunaga,N.,Teramura,T.,Onodera,Y.,Takehara,T.,Fukuda,K.,andHosoi,Y.(2010)Leukemiainhibitoryfactor(LIF)enhancesgermcelldifferentiationfromprimateembryonicstemcells.CellReprogram.12:369-376.
- MetcalfD.(2003)Theunsolvedenigmasofleukemiainhibitoryfactor.StemCells21:5-14.
- Williams,R.L.,Hilton,D.J.,Pease,S.,Willson,T.A.,Stewart,C.L.,Gearing,D.P.,Wagner,E.F.,Metcalf,D.,Nicola,N.A.,andGough,N.M.(1988)Myeloidleukemiainhibitoryfactormaintainsthedevelopmentalpotentialofembryonicstemcells.Nature336:684-687.
- Metcalf,D.,Hilton,D.J.,andNicola,N.A.(1988)Clonalanalysisoftheactionsofthemurineleukemiainhibitoryfactoronleukemicandnormalmurinehemopoieticcells.Leukemia2:216-221.
AdditionalPublications
- Ying,Q.,Tai,C.Gbx2,aLIF/Stat3target,promotesreprogrammingtoandretentionofthepluripotentgroundstate.JournalofCellScience126,1093–1098.(2013)
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