ProductOverview
TransformingGrowthFactorbeta1(TGF-β1)isamemberoftheTGF-βsuperfamilyofmultifunctionalpolypeptidegrowthfactorsthatincludesbonemorphogeneticproteins(BMPs),Activin,andNodal.TGF-β1bothhelpsmaintainpluripotencyinhumanembryonicstem(hES)cellsandinducesdifferentiation1,2.Italsoinducesepithelial-mesenchymaltransitionandsuppressesproliferationanddifferentiationofseveraltypesoflymphocytes,thusplayingimportantrolesinhumancancers3.StemfactorTGF-β1isa25kDahomodimericrecombinantproteinexpressedinhumanHEK293cellsandcomposedoftwoidentical112aminoacidpolypeptidechainslinkedbyasingledisulfidebond.
ProductSpecifications
Size
5μg
Source
StemfactorTGF-β1wasexpressedinandpurifiedfromhumanHEK293cells.
Formulation
Lyophilizedfromsterile-filtered50mMNaOAc,pH4.5,200mMNaCl.
AminoAcidSequence
ALDTNYCFSSTEKNCCVRQLYIDFRKDLGWKWIHEPKGYHANFCLGPCPYIWSLDTQYSKVLALYNQHNPGASAAPCCVPQALEPLPIVYYVGRKPKVEQLSNMIVRSCKCS
UniprotAccessionNumber
P01137,residues279-390.
Purity
Greaterthan95%bySDS-PAGEanalysis
EndotoxinLevel
Lessthan1.0EU/μgofTGF-β1asdeterminedbytheLALmethod.
BIOLOGicActivity
TheED50islessthan0.2ng/mlasdeterminedbyitsABIlitytoinhibitthedose-dependentinhibitionofIL-4inducedproliferationofmouseHT-2cells.
StorageandStability
StemfactorTGF-β1isshippedatroomtemperature.LyophilizedTGF-β1isstableforupto6monthsfromdateofreceiptwhenstoredat-20°Cto-80°C.ReconstitutedTGF-β1,atconcentrationsgreaterthanorequalto0.1mg/ml,isstableforupto3monthswhenstoredat-20°Candupto6monthswhenstoredat-80°C.
Reconstitution
CentrifugebrieflyandthenreconstituteTGF-β1in4mMHCltoyieldastocksolutionofnolessthan0.1mg/ml.Avoidfreeze-thawcyclesasitcanresultinlossofactivity.
SpecificationSheets
- 03-0004SpecificationsSheet
SafetyDataSheets
- 03-0004SafetyDataSheet
References
- Watabe,T.,andMiyazono,K.(2009)RolesofTGF-betafamilysignalinginstemcellrenewalanddifferentiation.CellRes.19:103-115.
- Yang,Z.,Sui,L.,Toh,W.S.,Lee,E.H.,andCao,T.(2009)Stage-dependenteffectofTGF-beta1onchondrogenicdifferentiationofhumanembryonicstemcells.StemCellsDev.18:929-940.
- Pardali,K.,andMoustakas,A.(2007)ActionsofTGF-betaastumorsuppressorandpro-metastaticfactorinhumancancer.BiochimBiophysActa.1775:21-62.
AdditionalPublications
- Wang,B.,Miyagoe-Suzuki,Y.,Yada,E.,Ito,N.,Nishiyama,T.,Nakamura,M.,Ono,Y.,Motohashi,N.,Segawa,M.,Masuda,S.,Takeda,S.ReprogrammingEfficiencyandQualityofInducedPluripotentStemCells(iPSCs)GeneratedfromMuscle-derivedFibroblastsofMDXMiceatDifferentAges.PLOSCurrentsMuscularDystrophy.2011Oct27.Edition1.doi:10.1371/currents.RRN1274.
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